OCCURRENCE OF CRYPTOSPORIDIUM SP. IN DOGS AND CATS FROM CURITIBA AND ITS METROPOLITAN AREA

Authors

  • Martha Grecca UFPR
  • Vanete Thomaz-Soccol UFPR
  • Magda C Costa Ribeiro UFPR
  • Jessé Henrique Truppel 2- Centro de Controle de Zoonoses, Departamento de Vigilância Sanitária, Secretária Municipal de Saúde, Prefeitura de Araucária, Rua Pedro Druszcz, 111 - CEP: 83702-080 – Araucária, Paraná, Brasil
  • Juliana Tracz Pereira 3- Instituto Federal do Paraná – campus Paranaguá
  • Silvia C Osaki 4- Universidade Federal do Paraná – Campus Palotina – Rua Pioneiro, 2153 – CEP 85950-000 – Palotina, Paraná, Brasil

DOI:

https://doi.org/10.5380/avs.v18i3.29522

Keywords:

Cryptosporidium sp., molecular diagnosis, nested PCR, 18SSU rDNA gene

Abstract

The present study was carried out with the aim of assess the occurrence of Cryptosporidium sp. infection in dogs and cats in Curitiba and its metropolitan area, state of Paraná, Brazil. Techniques employed to detect the protozoan in fecal samples were: staining by Ziehl-Neelsen for oocysts search and nested polymerase chain reaction (nPCR) targeting the 18SSU rDNA gene. To attempt the proposed aim, 91 feces samples of dogs and 25 of cats were collected and analyzed. Ziehl-Neelsen technique was unable to detect any oocyst in both groups analyzed, showing a very low sensitivity. Results of nPCR showed an infection rate of 13.2% (12/91) and 4% (1/25) in dogs and cats respectively.  The implications of these epidemiological data are discussed in this work.

Author Biographies

Martha Grecca, UFPR

Mestranda do Programa de Microbiologia, Parasitologia e Patologia

Magda C Costa Ribeiro, UFPR

Prof Adjunto do Departamento de Patologia Básica

Jessé Henrique Truppel, 2- Centro de Controle de Zoonoses, Departamento de Vigilância Sanitária, Secretária Municipal de Saúde, Prefeitura de Araucária, Rua Pedro Druszcz, 111 - CEP: 83702-080 – Araucária, Paraná, Brasil

Médico Veterinário Centro de Controle de Zoonoses, Departamento de Vigilância Sanitária, Secretária Municipal de Saúde, Prefeitura de Araucária,

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Published

2013-05-23

How to Cite

Grecca, M., Thomaz-Soccol, V., Costa Ribeiro, M. C., Truppel, J. H., Tracz Pereira, J., & Osaki, S. C. (2013). OCCURRENCE OF CRYPTOSPORIDIUM SP. IN DOGS AND CATS FROM CURITIBA AND ITS METROPOLITAN AREA. Archives of Veterinary Science, 18(3). https://doi.org/10.5380/avs.v18i3.29522

Issue

Vol. 18 No. 3 (2013)

Section

Preventive Veterinary Medicine

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