Efficacy of Autologous Serum in Surgical Wound Healing of Dogs (Canis lupus familiaris)
DOI:
https://doi.org/10.5380/avs.v31i2.101779Palavras-chave:
Autologous serum, modified Bates-Jensen Wound Assessment Tool, wound healingResumo
This study evaluated the efficacy of autologous serum (AS) in promoting the healing of primarily closed abdominal surgical wounds in female dogs using a modified Bates-Jensen Wound Assessment Too (BWAT). Thirty dogs were equally divided into Control, Treatment 1 (once-daily AS), and Treatment 2 (Twice-daily AS) groups. Wounds were assessed every other day over a 10-day postoperative period, focusing on parameters such as epithelialization, exudate, skin color, edema, necrosis, and wound edges. Findings revealed a strong positive correlation between AS application frequency and rate of epithelialization, with treatment 2 showing significant difference compared to other groups. Repeated measures ANOVA confirmed a highly significant difference among groups (p < 0.001). These results indicate that autologous serum not only accelerates wound healing but also promotes a more efficient inflammatory response without increasing the risk of complications. This study highlights Autologous Serum as biologically active, cost-effective adjunct to conventional wound management in veterinary surgery, offering practical benefits for clinical application.
Referências
Alfonso, I. (2024). Dogs in Philippine History. Philippines Historical Association, Project Saysay, Alaya Publishing.
Aiello, S. E., & Moses, M. A. (2016). The Merck Veterinary Manual. John Wiley & Sons.
Altoé, L. S., Alves, R. S., Sarandy, M. M., Morais-Santos, M., Novaes, R. D., & Gonçalves, R. V. (2019). Does antibiotic use accelerate or retard cutaneous repair? A systematic review in animal models. PLOS ONE, 14(10), e0223511. https://doi.org/10.1371/journal.pone.0223511
Anitua, E., Muruzabal, F., Tayebba, A., Riestra, A., Pérez, V. L., Merayo-Lloves, J., & Orive, G. (2015). Autologous serum and plasma rich in growth factors in ophthalmology: Preclinical and clinical studies. Acta Ophthalmologica, 93(8), e605-e614. https://doi.org/10.1111/aos.12710
Buid, G. (2023). The Philippines: Steadily growing demand in this up- and-coming market - Go-to resource for the global pet industry | GlobalPETS. Go-to Resource for the Global Pet Industry | GlobalPETS. https://globalpetindustry.com/article/philippines-steadily-growing-demand-and-coming-market
Bloemen, M., Boekema, B., Vlig, M., Van Zuijlen, P., & Middelkoop, E. (2012). Digital image analysis versus clinical assessment of wound epithelialization: A validation study. Burns, 38(4), 501–505. https://doi.org/10.1016/j.burns.2012.02.003
Bukowiecki, A., Hos, D., Cursiefen, C., & Eming, S. A. (2017). Wound-Healing studies in cornea and skin: parallels, differences and opportunities. International Journal of Molecular Sciences, 18(6), 1257. https://doi.org/10.3390/ijms18061257
Charernsriwilaiwat, N., Opanasopit, P., Rojanarata, T., & Ngawhirunpat, T. (2012). Lysozyme-loaded, electrospun chitosan-based nanofiber mats for wound healing. International Journal of Pharmaceutics, 427(2), 379–384. https://doi.org/10.1016/j.ijpharm.2012.02.010
Chhabra, S., Chhabra, N., Kaur, A., & Gupta, N. (2017). Wound Healing Concepts in Clinical Practice of OMFS. Journal of Maxillofacial and Oral Surgery, 16(4), 403–423. https://doi.org/10.1007/s12663-016-0880-z
Cortegiani, A. (2022). Use of autologous conditioned serum (ACS-ORTHOKINE) for osteoarthrosic chronic pain and facet joint syndrome: a prospective observational study. https://www.semanticscholar.org/paper/Use-of-autologous-conditioned-serum-(ACS-ORTHOKINE)-Cortegiani
De Pietro, M. (2017). Surgical Wound. Healthline. https://www.healthline.com/health/surgical-wound
Evans, C. H., Chevalier, X., & Wehling, P. (2016). Autologous conditioned serum. Physical Medicine and Rehabilitation Clinics of North America, 27(4), 893–908. https://doi.org/10.1016/j.pmr.2016.06.003
Fossum, T. W. (2013). Small Animal Surgery Textbook - E-Book. Elsevier Health Sciences.
Grey, J. E., Enoch, S., & Harding, K. G. (2006). ABC of wound healing: Wound assessment. BMJ: British Medical Journal, 332(7536), 285-288. https://doi.org/10.1136/bmj.332.7536.285
Gurtner, G. C., Werner, S., Barrandon, Y., & Longaker, M. T. (2008). Wound repair and regeneration. Nature, 453(7193), 314–321. https://doi.org/10.1038/nature07039
Guo, S., & DiPietro, L. A. (2010). Factors affecting wound healing. Journal of Dental Research, 89(3), 219–229. https://doi.org/10.1177/0022034509359125
Haber, M., Cao, Z., Panjwani, N., Bedenice, D., Li, W. W., & Provost, P. J. (2003). Effects of growth factors (EGF, PDGF-BB and TGF-beta 1) on cultured equine epithelial cells and keratocytes: implications for wound healing. Veterinary ophthalmology, 6(3), 211–217. https://doi.org/10.1046/j.1463-5224.2003.00296.x
Harris, C., Bates‐Jensen, B. M., Parslow, N., Raizman, R., Singh, M., & Ketchen, R. (2010). Bates-Jensen Wound Assessment Tool. Journal of Wound, Ostomy, and Continence Nursing/Journal of WOCN, 37(3), 253–259. https://doi.org/10.1097/won.0b013e3181d73aab
Heal, C., Banks, J., Lepper, P. D., Kontopantelis, E., & Van Driel, M. (2016). Topical antibiotics for preventing surgical site infection in wounds healing by primary intention. The Cochrane Library, (11). https://doi.org/10.1002/14651858.cd011426.pub2
HealthforAnimals. (2022). Global trends in the PET population - HealthforAnimals. https://www.healthforanimals.org/reports/pet-care-report/global-trends-in-the-pet-population/
Herman, T. F. (2023). Wound classification. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK554456/
Higuchi, A. (2018). Autologous serum and serum components. Investigative Ophthalmology & Visual Science, 59(14), DES121. https://doi.org/10.1167/iovs.17-23760
Horváthy, D. B., Simon, M., Schwarz, C., Masteling, M., Vácz, G., Hornyák, I., & Lacza, Z. (2016). Serum albumin as a local therapeutic agent in cell therapy and tissue engineering. BioFactors, 43(3), 315–330. https://doi.org/10.1002/biof.1337
Jee, C., Eom, N., Jang, H., Jung, H., Choi, E., Won, J., Hong, I., Kang, B., Jeong, D. W., & Jung, D. (2016). Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs. Journal of Veterinary Science, 17(1), 79. https://doi.org/10.4142/jvs.2016.17.1.79
Jee, D., Park, S. H., Kim, M. S., & Kim, E. C. (2014). Antioxidant and inflammatory cytokine in tears of patients with dry eye syndrome treated with Preservative-Free versus preserved eye drops. Investigative Ophthalmology & Visual Science, 55(8), 5081. https://doi.org/10.1167/iovs.14-14483
Johnston, S. A., & Tobias, K. M. (2017). Veterinary Surgery: Small Animal Expert Consult - E-BOOK: Veterinary Surgery: Small Animal Expert Consult - E-BOOK. Elsevier Health Sciences.
Kamel, C., McGahan, L., Mierzwinski-Urban, M., & Embil, J. (2011). Classification of surgical wounds. Preoperative Skin Antiseptic Preparations and Application Techniques for Preventing Surgical Site Infections: A Systematic Review of the Clinical Evidence and Guidelines - NCBI Bookshelf. https://www.ncbi.nlm.nih.gov/books/NBK174534/
Kim, N., Choi, K. U., Lee, E., Lee, S., Oh, J., Kim, W. K., Woo, S. H., Kim, D. Y., Kim, W. H., & Kweon, O. K. (2020). Therapeutic effects of platelet derived growth factor overexpressed-mesenchymal stromal cells and sheets in canine skin wound healing model. Histology and histopathology, 35(7), 751–767. https://doi.org/10.14670/HH-18-196
Landa, D. L., Van Dishoeck, A., Steyerberg, E. W., & Hovius, S. E. (2014). Quality of measurements of acute surgical and traumatic wounds using a digital wound‐analysing tool. International Wound Journal, 13(5), 619–624. https://doi.org/10.1111/iwj.12330
Lin, H., Lin, S., & Chang, F. (2018). Effects of autologous serum eye drops for treatment of keratoconjunctivitis sicca in dogs. Taiwan Veterinary Journal, 44(3), 111–117. https://doi.org/10.1142/s1682648518500051
Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews/Bioinformatics Trends/Pharmacognosy Review, 4(8), 118. https://doi.org/10.4103/0973-7847.70902
Mich, P. M., Hellyer, P. W., Kogan, L. R., & Schoenfeld-Tacher, R. (2010). Effects of a pilot training program on veterinary students’ pain knowledge, attitude, and assessment skills. Journal of Veterinary Medical Education, 37(4), 358–368. https://doi.org/10.3138/jvme.37.4.358
Multidisciplinary Digital Publishing Institute (MDPI). (2022). Veterinary Surgery. https://www.mdpi.com/journal/vetsci/sections/Veterinary_Surgery
Polcz, M., & Barbul, A. (2019). The role of vitamin A in wound healing. Nutrition in Clinical Practice, 34(5), 695–700. https://doi.org/10.1002/ncp.10376
Releford, B. (2022). Differentiate between traditional and advanced wound care. The Wound Pros. https://www.thewoundpros.com/post/traditional-wound-care-vs-advanced-wound-care-evolution-of-the-industry
Sarabahi, S. (2012). Recent advances in topical wound care. Indian Journal of Plastic Surgery, 45(02), 379–387. https://doi.org/10.4103/0970-0358.101321
Semeraro, F., Forbice, E., Braga, O., Bova, A., Di Salvatore, A., & Azzolini, C. (2014). Evaluation of the efficacy of 50% autologous serum eye drops in different ocular surface pathologies. BioMed Research International, 14(1), 1–11. https://doi.org/10.1155/2014/826970
Scalise, A., Calamita, R., Tartaglione, C., Pierangeli, M., Bolletta, E., Gioacchini, M., Gesuita, R., & Di Benedetto, G. (2015). Improving wound healing and preventing surgical site complications of closed surgical incisions: a possible role of Incisional Negative Pressure Wound Therapy. A systematic review of the literature. International Wound Journal, 13(6), 1260–1281. https://doi.org/10.1111/iwj.12492
Seria, E., Tagliaferro, S. S., Cutajar, D., Galdies, R., & Felice, A. E. (2021). Immunoglobulin G in Platelet-Derived Wound Healing Factors. BioMed Research International, 1–16. https://doi.org/10.1155/2021/4762657
Spear, M. (2012). Wound Exudate—The Good, the bad, and the ugly. Plastic Surgical Nursing, 32(2), 77–79. https://doi.org/10.1097/psn.0b013e318256d638
Takayama, Y., & Aoki, R. (2012). Roles of lactoferrin on skin wound. Biochemistry and Cell Biology, 90(3), 497–503. https://doi.org/10.1139/o11-054
Tavassi, A. M. C. (2014). Wound healing effects of a Lipocalin-Derived peptide. Journal of Clinical Toxicology, 4(2). https://doi.org/10.4172/2161-0495.10001
Tonglairoum, P., Ngawhirunpat, T., Rojanarata, T., & Opanasopit, P. (2014). Lysozyme-immobilized electrospun PAMA/PVA and PSSA-MA/PVA ion-exchange nanofiber for wound healing. Pharmaceutical Development and Technology, 20(8), 976–983. https://doi.org/10.3109/10837450.2014.954726
Vazirani, J., Sridhar, U., Gokhale, N., Doddigarla, V. R., Sharma, S., & Basu, S. (2023). Autologous serum eye drops in dry eye disease: Preferred practice pattern guidelines. Indian Journal of Ophthalmology, 71(4), 1357–1363. https://doi.org/10.4103/ijo.ijo_2756_22
Winkler, K. P. (2023). Wound management. MSD Veterinary Manual. https://www.msdvetmanual.com/special-pet-topics/emergencies/wound-management
Xu, P., Wu, Y., Zhou, L., Yang, Z., Zhang, X., Hu, X., Yang, J., Wang, M., Wang, B., Luo, G., He, W., & Cheng, B. (2020). Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization. Burns & Trauma, 8, tkaa028. https://doi.org/10.1093/burnst/tkaa028
Yang, Y., Zhang, C., Cao, Y., Qu, Y., Li, T., Yang, T., Geng, D., & Sun, Y. (2020). Bidirectional regulation of i-type lysozyme on cutaneous wound healing. Biomedicine & Pharmacotherapy, 131, 110700. https://doi.org/10.1016/j.biopha.2020.110700
Zhang, S., Tan, H., Cheng, X., Dou, X., Fang, H., Zhang, C., Yang, G., Yang, H., Zhao, Y., Feng, T., Fan, H., & Sha, W. (2024). Autologous platelet-rich fibrin enhances skin wound healing in a feline trauma model. BMC Veterinary Research, 20(1), 504. https://doi.org/10.1186/s12917-024-04358-4
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