Cryopreservation of dog spermatozoa: effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination

Authors

  • I. W. Santos
  • L. C. Binsfeld
  • R. R. Weiss
  • L. E. Kozicki
  • R. Rochadelli
  • M. V. Resende
  • V. F. M. Hossepian de Lima

DOI:

https://doi.org/10.5380/avs.v12i2.9909

Keywords:

Bitch, oestrus, extenders, semen, cadela, estro, diluidor

Abstract

The objective of the present study was
to compare the in vitro and in vivo profile of frozen
dog semen with Tris-bovine serum albumin (TB)
and Tris-egg yolk (TE) extenders. Twenty dogs
were used as donors. Each dog was stimulated by
penile massage and only the sperm-rich fraction
was collected weekly until 40 ejaculates were obtained.
After macroscopic and microscopic analysis,
equal parts of each ejaculate were diluted with TB
and TE by the one-step method at 37 ºC. The semen
was added to 0.5-mL French straws which presented
normal characteristics before freezing and
after thawing. Acrosomal integrity was evaluated by
double Trypan blue-Giemsa staining, in which alive
intact (LI), alive reacted (LR), dead intact (DI) and
dead reacted (DR) spermatozoa, were identified
by the time of thawing and up to 4 h of incubation
at 39 °C, the TE being significantly superior to TB
(P<0,01) in the LI and LR variables. The TB being
significantly superior to TE (P<0,01) in the DR variable.
Female dogs in natural heat were submitted to
artificial insemination, 20 receiving TE-semen and
20 receiving TB-semen with the Osiris probe (IMV,
L’Aigle, France) and the numbers indicate that TE
was significantly better than TB (P<0,01) to pregnancy
rate and number of puppies/delivery. We
concluded from this study, that TE was better than
TB, because this, induced an early acrossome reaction
in dog’s sperm.

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Published

2007-12-06

How to Cite

Santos, I. W., Binsfeld, L. C., Weiss, R. R., Kozicki, L. E., Rochadelli, R., Resende, M. V., & Hossepian de Lima, V. F. M. (2007). Cryopreservation of dog spermatozoa: effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination. Archives of Veterinary Science, 12(2). https://doi.org/10.5380/avs.v12i2.9909

Issue

Vol. 12 No. 2 (2007)

Section

Preventive Veterinary Medicine

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