Quantitative analyses of polyphenol extracts from inflorescences of Musa x paradisiaca against free-living stages of Haemonchus contortus

Authors

DOI:

https://doi.org/10.5380/avs.v31i2.101782

Keywords:

Phytotherapy, Biofriendly products, principal components, gastrointestinal parasites, in vitro assays

Abstract

Extracts from Musa x paradisiaca inflorescences were chemically characterized and tested in vitro on ruminant parasite egg hatch (EHT) and larval migration (LMT) tests. The aqueous (EHW-I) and hydroalcoholic (EOH-I) extracts of the plant were analyzed by using colorimetric assays, high-performance liquid chromatography (HPLC), and electrospray tandem mass spectrometry (ESI-MS/MS). EHW-I and EOH-I had similar phenolic contents. However, EOH-I had a higher flavonoid content than EHW-I. Both extracts presented low contents of condensed tannins. HPLC analysis showed that EHW-I contained phenolic acids (chlorogenic acid ˃ coumaric acid > ferulic acid), other than rutin (glycosylated flavonoid). EOH-I contained rutin as the predominant compound. EHW-I and EOH-I showed an inhibitory effect on EHT, reaching almost 100% at a concentration of 1000 µg/mL. The inhibitory concentration of 50% (IC50) of egg hatching was 62.58 and 137.79 µg/mL of EHW-I and EOH-I, respectively. Based on the quantification data of phenolic and flavonoid compounds in the extracts, chlorogenic acid and rutin were selected for the EHT. Chlorogenic acid presented high activity, with an IC50 of 17.55 µg/mL. Rutin, however, did not affect egg hatching under the conditions tested. It can be inferred that chlorogenic acid may be a candidate with ovicidal activity. EHW-I and EOH-I did not affect the migration of third-stage nematode larvae, which may be related to the low tannin content. The inflorescences of Musa x paradisiaca can be further explored to develop a plant-derived anthelmintic product for ruminants and other animals.

Author Biographies

Melina Seyfried , Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-900, Curitiba, PR, Brazil,

She holds a Bachelor’s degree in Biological Sciences from Universidade Positivo (2008), a specialization in Cell and Tissue Biology from the Federal University of Paraná (2010), a Master’s degree (2014), and a Ph.D. (2018) in Sciences (Biochemistry) from the Federal University of Paraná. Her research experience focuses on the isolation and chemical and structural characterization of primary and secondary metabolites from medicinal plants and/or species of economic interest.

Vanessa Staldoni-Oliveira , Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-900, Curitiba, PR, Brazil,

Bachelor's degree in Biological Sciences (full degree) from the Federal University of Rio Grande do Sul. Master's degree in Biochemistry from the Federal University of Paraná. PhD in Biochemistry from the Federal University of Santa Catarina. Specialist in Permaculture from the Federal University of Santa Catarina. Postdoctoral studies in Biochemistry from the University of Coimbra, Portugal. Experience in phytochemistry, ecotoxicology, oxidative stress, male reproductive system dysfunction, urban agriculture, and teaching natural sciences in elementary schools.

Luciano Henrique Campestrini , Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-900, Curitiba, PR, Brazil,

He holds a degree in Pharmaceutical Sciences (2003) and a specialization in Food Technology from the Federal University of Santa Catarina (2006). He holds a Master's degree in Biotechnology with an emphasis on Agroforestry (2007) from the same university. He holds a PhD in Biochemistry from the Federal University of Paraná-UFPR (2013). He completed postdoctoral internships in Food Biochemistry at ESALQ - USP (2014-2016) and in Biotechnology of Edible and Medicinal Fungi at the National Institute for Amazonian Research - INPA (2020-2022). He has experience in in vitro culture of plant cells and tissues, biochemistry of plant and fungal secondary metabolites, and carbohydrate chemistry, working mainly on the following topics: Liquid and gas chromatography coupled with mass spectrometry, infrared spectroscopy, NMR, and biological activities of polysaccharides, glycoconjugates, and natural products.

Andressa Soldera-Silva , Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-900, Curitiba, PR, Brazil,

She graduated in Biological Sciences from Universidade Positivo in 2011. She completed her final project at the Glycojugates Laboratory of the Department of Biochemistry and Molecular Biology at UFPR, entitled "Antioxidant Activity of Polysaccharides from the Fruit of Physallis peruviana." She also assisted in experiments conducted there in the cell culture area. She also completed her master's degree in March 2014, also at the Glycojugates Laboratory of the Department of Biochemistry and Molecular Biology at UFPR, with her dissertation entitled "Structural Characterization and Biological Activity of Polysaccharides from Physalis peruviana L." She completed her doctorate at the same research laboratory and her thesis focused on various medicinal plants with antiparasitic effects in sheep.

Fernanda Bovo , Department of Pharmacy, Pharmacy School Laboratory Federal University of Paraná, 80210-170, Curitiba, PR, Brazil

Graduated in Pharmacy with specialization in Clinical Analysis from the State University of Londrina (2005). Master in Experimental Pathology from the State University of Londrina - beginning in (2007). PhD in Pharmaceutical Sciences from the Federal University of Paraná (2013). Post-doctorate at the Federal University of Paraná (2013-2015; and 2015-2016). Degree in Biological Sciences (2017). She was an undergraduate and graduate professor at the State University of the Central West (2007-2013); Professor at the State Department of Education (SEED) (2016-2019). Currently, she is a civil servant at UFPR assigned to the Laboratory School of Clinical Analysis (UFPR) and, since 2019, a professor of Pharmacy, Biomedicine, Dentistry, Physiotherapy, Nutrition and Biological Sciences courses at UNICURITIBA. He has experience in research in basic and clinical immunology, medicinal plants and product development.

Iara José de Messias-Reason , Department of Medical Pathology, Federal University of Paraná, 80060-900, Curitiba, PR, Brazil

She holds a Bachelor’s degree in Biochemical Pharmacy from the State University of Ponta Grossa, and a Master’s and Ph.D. in Natural Sciences (with an emphasis in Immunogenetics) from Medizinische Hochschule Lübeck, Germany. She completed postdoctoral training at the University of Cincinnati, USA (1986), at the Institute of Immunology of the University of Heidelberg, Germany (1995), and at the Institute of Tropical Medicine of the University of Tübingen, Germany (2005–2006). She is currently a Full Professor in the Department of Medical Pathology at the Federal University of Paraná. Her research experience lies in Immunology, with emphasis on Immunogenetics and Molecular Immunopathology, focusing primarily on the molecular basis of the host innate immune response in infectious and autoimmune diseases, particularly involving the complement system. More recently, she has initiated a new line of research on the immunomodulatory activities of natural products.

Jonas Augusto Rizzato Paschoal , Department of Physics and Chemistry, University of São Paulo, 14040-903, Ribeirão Preto, SP, Brazil

He is a Full Professor - Associate III at FCFRP/USP and currently Vice-Head of the Department of Biomolecular Sciences. He coordinates the FCFRP Graduate Program in Toxicology and is also accredited as a full advisor for the Graduate Program in Pharmaceutical Sciences at the same school. He is an associate member of the Brazilian Society of Toxicology (SBTox), through which he coordinates the Specialized Group in Food Toxicology (GETox-Alimentos). He is also an associate member of the Brazilian Association of Pharmaceutical Sciences (ABCF). He conducts research in Applied Analytical Chemistry, developing bioanalytical methods for organic compounds in various types of biological samples. He has experience with modern chromatographic separation instrumentation (UPLC-DAD, FLD, and primarily UPLC-MS/MS). He conducts in vivo studies in food-producing animals. He collaborates on various national and international scientific projects, including planning, development, and discussion of results involving analytical chemistry. He was a FAPESP Young Researcher Fellow (2013-2018). He is a member of the Roster of Experts 2023-2027 of the Expert Committee on Food Additives (JECFA) of the Food and Agriculture Organization of the United Nations and the World Health Organization (FAO/WHO). He was a member of the Working Group created by Anvisa to review the Resolution on the establishment of Maximum Residue Limits for veterinary drugs in foods of animal origin.

Selma Faria Zawadzki , Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-900, Curitiba, PR, Brazil,

She holds a degree in Biological Sciences from the Federal University of Paraná (1988) and a PhD in Biochemistry from the Federal University of Paraná and the University of Stirling, Scotland. She is currently a Full Professor at the Federal University of Paraná. She has experience in Biochemistry, with an emphasis on Carbohydrates, working mainly in the study of the chemical structure of polysaccharides from medicinal plants and the characterization of secondary metabolites of medicinal plants.

Marcelo Beltrão Molento , Department of Veterinary Medicine, Federal University of Paraná, 80035-050, Curitiba, PR, Brazil

Dr Molento holds a degree in Veterinary Medicine from the University of Alfenas (UNIFENAS, 1989) and a Ph.D. in Parasitology from McGill University, Canada (2000). He is a Full Professor at the Federal University of Paraná, Coordinator of the Laboratory of Veterinary Clinical Parasitology, and a CNPq Research Fellow since 2011. He also coordinates the postgraduate program Global Health at UFPR, which aims to train critical thinkers to address diverse global challenges. He collaborates with several research and funding institutions in Brazil (PR, BA, SC, SP, MS, RO, CE, MA, PE, ES) and internationally (Argentina, Colombia, Cuba, USA, Uruguay, Belgium, Italy, and the Netherlands). He serves as a consultant for the FAO/UN, WHO, and WOAH (formerly OIE) on parasitic diseases involving human–animal interactions and parasite resistance. His main scientific objective is to develop technologies based on the Integrated Parasite Control System (SICOPA) to improve animal, human, and environmental health. SICOPA encompasses studies on resistance in veterinary parasites (e.g., Haemonchus contortus), diagnosis and maintenance of anthelmintic efficacy, and the development of novel control strategies, including phytotherapy and ethnoveterinary approaches. He is also a promoter of the FAMACHA method and works on the development of antiparasitic products, as well as in vitro and molecular diagnostic techniques, including research on Fasciola hepatica and several zoonoses (e.g., leptospirosis and Brazilian spotted fever). He coordinates the extension program PARAVET and is the curator of the Biological Collection of Veterinary Parasitic Organisms at UFPR. He is a founding member of the Wildlife Health Information Center (CISS), coordinated by FIOCRUZ–RJ with support from the World Bank, and of the National Institute of Science and Technology for Livestock Information (INCT-Pecuária), based at UFMG in Belo Horizonte. He also serves on the editorial boards of Veterinary Parasitology and the Brazilian Journal of Veterinary Parasitology (RBPV) and is a reviewer for more than 25 scientific journals.

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2026-05-14

How to Cite

Maurer, J. B. B., Seyfried , M., Staldoni-Oliveira , V., Campestrini , L. H., Soldera-Silva , A., Bovo , F., … Beltrão Molento , M. (2026). Quantitative analyses of polyphenol extracts from inflorescences of Musa x paradisiaca against free-living stages of Haemonchus contortus. Archives of Veterinary Science, 31(2). https://doi.org/10.5380/avs.v31i2.101782

Issue

Vol. 31 No. 2 (2026): Three Decades of Science: The Vanguard of Archives of Veterinary Science

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Veterinary Parasitology

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