Natural <i>Clostridium botulinum</i> type “c” toxicosis in a group of chicken

Abstract

Clostridium botulinum causative of
toxic infections due to toxin ingestion previously
formed, occur in several ingestion species, mainly
birds. In a poultry farm, located at São Paulo State,
more than 3,000 birds have been attacked by this
toxic infections, when the birds showed motionless,
loss of weight, accelerated, breathing, resulting in
death of the birds. The blood harvest for attainment
of the serum and later necropsy was carried out
throwgh the following samples: liver, gizzard, crop,
feed, water and litter. After that, the toxin was
extracted by gelatin-phosphate buffer and inoculation
in mice, isolation of the agent in Blood ágar and
Reinforced Clostridium ágar and neutralization of
toxin determine its type. The inoculation in mice
showed positive results in samples of liver, gizzad,
crop and symptoms like was waist, and death of
the birds by limp paralysis. The colonies that have
been isolated, suspected of Clostridium botulinum
showed expected results and further analysis
revealed positive results to botulinical toxin type C.
The farms have to pay attention in the routine jobs,
choicer, and elimination of carcass because they
are essential to keep this problem away.