In vitro anthelmintic activity of Polygonum acre (Linnaeus, 1754) extracts against the ruminant nematode Haemonchus contortus (Rudolphi, 1803)

Authors

  • Melina Seyfried Federal University of Paraná, Curitiba, PR
  • Andressa Soldera-Silva Federal University of Paraná, Curitiba, PR
  • Luciano Henrique Campestrini National Institute for Amazonian Research - INPA, Manaus, AM
  • Diogo Alexandre Siebert Federal University of Santa Catarina, Florianópolis, SC
  • Luciano Vitali Federal University of Santa Catarina, Florianópolis, SC
  • Gustavo Amadeu Micke Federal University of Santa Catarina, Florianópolis, SC
  • Selma Faria Zawadzki-Baggio Federal University of Paraná, Curitiba, PR
  • Marcelo Beltrão Molento Federal University of Paraná, Curitiba, PR https://orcid.org/0000-0003-0572-5628
  • Juliana Bello Baron Maurer Federal University of Paraná, Curitiba, PR https://orcid.org/0000-0001-6235-331X

DOI:

https://doi.org/10.5380/avs.v27i3.83890

Keywords:

egg hatch test, flavonoids, Haemonchus contortus, water pepper.

Abstract

The genus Polygonum exhibits a variety of biological and therapeutic properties. The goal of this study was to determine the in vitro anthelmintic activity of Polygonum acre extracts against the nematode Haemonchus contortus, characterizing the phenolic profile of these extracts. Aerial parts of P. acre were subjected to hot aqueous and hydroethanolic extractions obtaining EHW-PA (aqueous) and ETOH-P, (hydroethanolic), respectively. The phenolic content of the extracts was analyzed by using high-performance liquid chromatography with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) and colorimetric assays. The egg hatch test (EHT) and larval migration test (LMT) were used for anthelmintic activity assays. For comparison, chlorogenic acid and rutin were selected for the EHT. The HPLC-ESI-MS/MS analysis revealed the presence of isoquercitrin, myricitrin, quercetin, rosmarinic acid, rutin, and taxifolin in both extracts. EHW-PA and ETOH-PA showed an egg hatch inhibitory effect. EHW-PA had an IC50 of 442.24 µg/mL on the EHT, which was statistically lower (P ≤ 0.05) than ETOH-PA (IC50 = 703.60 µg/mL). Chlorogenic acid had an IC50 of 8.66 µg/mL. Rutin presented no effect over parasite eggs. EHW-PA and ETOH-PA did not show any larvicidal effect. Even though, there is a need of new tests, this study has determined the possibility to develop biofriendly compounds based on Polygonum spp. constituents against H. contortus.

Author Biographies

Melina Seyfried, Federal University of Paraná, Curitiba, PR

Graduated in Biological Sciences (Bachelor's Degree) from Universidade Positivo (2008), specialization in Cellular and Tissue Biology from Federal University of Paraná (2010) and Master's (2014) and Doctorate (2018) in Science (Biochemistry) from Federal University of Paraná. In the area of scientific research, he has experience in the area of isolation, chemical and structural characterization of primary and secondary metabolites of medicinal plants and/or of economic interest. In the analysis of the immunomodulatory action of plant extracts on cell culture of murine peritoneal macrophages and investigation of the fixation of the complement system, in vitro. In the study of in vitro antitumor activity of plant extracts on cell culture of the HeLa strain, as well as in vivo toxicity analysis on the minicrustacean Artemia salina. In research on the antioxidant property of plant extracts in different in vitro assays and the antiparasitic activity, in vitro, using eggs and larvae of Haemonchus contortus. Acted in online tutoring in the disciplines of general biochemistry, systemic biochemistry, cytology/cell biology, embryology, molecular biology, scientific methodology, applied chemistry and physical chemistry, Phyto cosmetics, Immunology, biosafety in the blended courses of Biomedicine, Aesthetics and Cosmetics, Nutrition, Physiotherapy and Physical Education. Teaching in the subjects of Environment and Ecology for the 100% EaD Environmental Management course. Expertise in the online disciplines Public Health, Environment, Psychology Applied to Health, Hormones and Scientific Methodology shared by several on-site courses. Acting as a teacher in the on-site classes of the Biochemistry discipline for the semi-attendance course on Nutrition. TCC advisor and board for face-to-face and blended Biomedicine.

Andressa Soldera-Silva, Federal University of Paraná, Curitiba, PR

Graduated in Biological Sciences from Universidade Positivo in 2011. She completed her course conclusion work at the Laboratory of Glycojugates of the Department of Biochemistry and Molecular Biology at UFPR, entitled "Antioxidant activity of polysaccharides from the fruit of Physallis peruviana". She also helped in experiments that took place in the laboratory, in the area of cell culture. In addition, he finished his master's degree in March 2014 also at the Laboratory of Glycojugates of the Department of Biochemistry and Molecular Biology at UFPR with his dissertation entitled "Structural characterization and biological activity of polysaccharides from Physalis peruviana L. She has a doctorate in this same research laboratory and has as the theme of his thesis different medicinal plants that have antiparasitic action in sheep.

Luciano Henrique Campestrini, National Institute for Amazonian Research - INPA, Manaus, AM

Graduated in Pharmaceutical Sciences (2003) and in Food Technology from the Federal University of Santa Catarina (2006). Master in Biotechnology with emphasis on agroforestry (2007) by the same University. He holds a PhD in Science-Biochemistry from the Federal University of Paraná-UFPR (2013). He held a post-doctoral internship in Food Biochemistry at ESALQ-USP (2014-2016). He is currently doing a post-doctoral internship at the National Institute for Research in the Amazon (INPA). He has experience in the field of in vitro culture of plant and animal cells and tissues and biochemistry, Biochemistry of Secondary Plant Metabolites and Carbohydrate Chemistry acting on the following subjects: Liquid and gas chromatography, Infrared spectroscopy, NMR and activities Polysaccharides, Glycoconjugates and Natural Products.

Diogo Alexandre Siebert, Federal University of Santa Catarina, Florianópolis, SC

Graduated in Pharmacy (2010), with specialization in clinical analysis, from Universidade Regional de Blumenau, master's degree in chemistry (2014), with an emphasis in the chemistry of natural products, from Universidade Regional de Blumenau and doctorate in chemistry, with an emphasis in analytical chemistry, by the Federal University of Santa Catarina (2019). She has experience in the fields of pharmacy, clinical analysis, natural products chemistry, analytical chemistry, chromatography and capillary electrophoresis.

Luciano Vitali, Federal University of Santa Catarina, Florianópolis, SC

Bachelor's Degree in Chemistry from the Federal University of Santa Catarina (2005), Master's Degree in Chemistry from the Federal University of Santa Catarina with a CNPq Scholarship (2008) and a PhD in Chemistry from the Federal University of Santa Catarina with a CNPq Scholarship (2012). Has experience in Chemistry, focusing on Analytical Chemistry, acting on the following subjects: metal adsorption using chitosan and modified chitosan, metal determination by Atomic Absorption Spectrometry, development of analytical methods by Capillary Electrophoresis using modified capillaries for applications in the analysis of drugs and biological samples, chemical analysis of drugs and foods by Liquid Chromatography coupled with Mass Spectrometry. He is a permanent professor at the Postgraduate Program in Chemistry at UFSC (PROEX-Capes, Grade 7) in the field of Analytical Chemistry and currently advises four doctoral students, three masters and one scientific initiation student.

Gustavo Amadeu Micke, Federal University of Santa Catarina, Florianópolis, SC

Graduated with a bachelor’s degree in Chemistry from the University of São Paulo (2000) and a PhD in Chemistry (Analytical Chemistry) [Sp-Capital] from the University of São Paulo (2004). He is currently Associate Professor IV at the Federal University of Santa Catarina. He has experience in the development of analytical methods, with an emphasis on capillary electrophoresis and liquid chromatography coupled with mass spectrometry, working mainly on the following topics: development of methods for the analysis of biodiesel, natural products, food, pharmaceutical and cosmetic inputs using simulation software. He works in the search for methods that achieve high analytical frequency using multiple injection strategies. Another line of research of interest is to develop analytical protocols to attest to the effectiveness of cosmetic products. He is currently deputy coordinator of the graduate program in Chemistry at UFSC.

Selma Faria Zawadzki-Baggio, Federal University of Paraná, Curitiba, PR

Selma Faria Zawadzki Baggio holds a bachelor’s degree in Biological Sciences from the Federal University of Paraná (1988) and a PhD in Biochemistry from the Federal University of Paraná and the University of Stirling, Scotland. She is currently a Full Professor at the Federal University of Paraná. She has experience in the field of Biochemistry, with an emphasis on Glycides, working mainly in the study of the chemical structure of medicinal plant polysaccharides and characterization of medicinal plant secondary metabolites.

Marcelo Beltrão Molento, Federal University of Paraná, Curitiba, PR

Prof. Molento is a Vet. Doctor, graduated from Universidade de Alfenas, UNIFENAS in 1989, PhD from the Institute of Parasitology from McGill University, Canada (2000). He is Associate Prof. at Universidade Federal do Paraná, Coordinator of Parasitic Diseases Laboratory and a researcher for CNPq. He is the Coordinator of the graduate course: GLOBAL HEALTH, UFPR that has the objective to form thinkers to face different worldwide scenarios. He is consultant for many research institutions in Brazil (PR, BA, SC, SP, MS, RO, CE, MA, PE, ES) and from Argentina, Colombia, Cuba, the EUA, Uruguay, Belgium and Holland. He is also a consultant to FAO/UN, WHO and OIE for parasitic diseases and the interface human-animal. His scientific objective is to develop technics using Sistema Integrado de Controle Parasitário, SICOPA, to improve life quality and welfare of animals, humans and the environment.

Juliana Bello Baron Maurer, Federal University of Paraná, Curitiba, PR

Graduated with a Degree in Biological Sciences from the Federal University of Paraná (1990), master of Science (Biochemistry, Enzymology) by the Federal University of Paraná (1993), Doctorate in Sciences (Biochemistry, carbohydrate Chemistry) from the Federal University of Paraná (1999), and Post-doctorate in the areas of Botany, Plant Physiology and Biochemistry, the University of Melbourne (School of Botany, Plant Cell Biology Research Centre), Australia (2005). She is currently a professor at the Federal University of Paraná. In research area, has expertise in structural and functional characterization of carbohydrates and arabinogalactan-proteins (AGPs). Currently, the main focus of research projects involves the chemical characterization and the investigation of the immunomodulatory activity against the human complement system of bioactive compounds (arabinogalactan-protein, pectins, and phenolic compounds) obtained from medicinal plants.

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Published

2022-09-12

How to Cite

Seyfried, M., Soldera-Silva, A., Campestrini, L. H., Siebert, D. A., Vitali, L., Micke, G. A., … Maurer, J. B. B. (2022). In vitro anthelmintic activity of Polygonum acre (Linnaeus, 1754) extracts against the ruminant nematode Haemonchus contortus (Rudolphi, 1803). Archives of Veterinary Science, 27(3). https://doi.org/10.5380/avs.v27i3.83890

Issue

Vol. 27 No. 3 (2022)

Section

Veterinary Parasitology

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