Sexing of in vitro produced bovine embryos: A review of methods with emphasis on PCR
DOI:
https://doi.org/10.5380/avs.v11i1.5630Keywords:
bovino, fertilização in vitro, sexagem, cattle, in vitro fertilization, sexing, PCRAbstract
The development of new animal reproduction biotechniques has been provided the obtaining of genetic improvement in cattle herds around the world. Recently, the ascension of the in vitro production (IVP) of bovine embryos provided important development in the technology of bovine embryos. Although being a consolidated technique, some studies show disproportion between the sexes of calves born by means of IVP, being the percentage of males higher than the theoretically expected rate of 50% for each sex. This inconvenient opened the perspective for the development of methods for sex determination for the embryos before the transference, once the production of females is desirable in economic terms. This revision will approach the advantages and disadvantages of the invasive and non-invasive methods developed for the sex determination in bovine embryos, with emphasis for PCR assay that is the technique that displays the best results.
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