Association between the serologic analysis and the polymerase chain reaction (PCR) in the diagnostic of the Mycoplasma hyopneumoniae infection in pigs during quarantine procedure

Authors

  • Danilo Leal Rocha Microvet
  • William Marcos Costa Microvet
  • Mayka Rabelo Microvet
  • José Lúcio dos Santos Microvet
  • Geraldo Camilo Alberton Universidade Federal do Paraná

DOI:

https://doi.org/10.5380/avs.v14i3.13632

Keywords:

biossegurança, PCR, pneumonia enzoótica, soroconversão, sorologia, biosecurity, enzootic pneumoniae, seroconversion, serology

Abstract

This study investigated through serologic analysis and polymerase chain reaction (PCR) the presence of Mycoplasma hyopneumoniae in reproductive gilts during quarantine proceeding. This study was realized in 300 Landrace x Large White gilts with 150 days of age in a quarantine unity in Parana in 2007.  These animals came from a single farm, considered free from Mycoplasma hyopneumoniae. The exams were made at Microvet laboratory located in Viçosa-MG. Clinical signs that suggest of respiratory problems were not seen, nor seroconversion was detected in 130 serologic analysis, performed in serum samples collected on days 0, 55 and 76 after housing, however, the Mycoplasma hyopneumoniae was detected through PCR (649 bp fragment of 16S gene) in tracheobronchiolar wash and pulmonary tissue from a gilt without conclusive result in serologic analysis in a sample collected 55 days after housing. Hence, association between serologic analysis and PCR was important to diagnose Mycoplasma hyopneumoniae during quarantine proceedings.

Author Biographies

Danilo Leal Rocha, Microvet

Médico Veterinário formado na UFV-MG no ano de 2001. Mestre em Patologia Veterinária pela UFPR em 2008. Atua na área de clínica e patologia de suínos pela Microvet.

William Marcos Costa, Microvet

Médico Veterinário formado pela UFV-MG. Atua na área de clínica e patologia de suínos pela Microvet

José Lúcio dos Santos, Microvet

Médico Veterinário formado pela UFMG em 1976, mestrado pela UFMG em 1981 e doutorado em ciência animal pela UFMG (1997). Atuou como professor adjunto da UFV entre 1997 a 2003, atua na área de microbiologia, biologia molecular, clínica e patologia de suínos.

Geraldo Camilo Alberton, Universidade Federal do Paraná

Médico veterinário formado pela UFPR em 1990, mestre pela UFPR (1996), doutorado pela UNESP (2000). Atua como Professor adjunto da UFPR na área de clínica e patologia de suínos

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How to Cite

Rocha, D. L., Costa, W. M., Rabelo, M., dos Santos, J. L., & Alberton, G. C. (2009). Association between the serologic analysis and the polymerase chain reaction (PCR) in the diagnostic of the Mycoplasma hyopneumoniae infection in pigs during quarantine procedure. Archives of Veterinary Science, 14(3). https://doi.org/10.5380/avs.v14i3.13632

Issue

Vol. 14 No. 3 (2009)

Section

Scientific Notes

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