Preliminary study on ozone hemotherapy for canine ischemic renal failure

Resumo

In dogs, kidney injury is a sudden deterioration in renal function that can be fatal. Ischemia, inflammation, nephrotoxin exposure, and infectious diseases are the most frequent causes. In several fields of regenerative medicine, ozone therapy has recently attracted considerable attention. Clinical studies have shown that ozone therapy is safe and effective, and that it reduces readmission rates for kidney injury in animal models of renal disorders. This study investigated the efficacy of ozone therapy in experimentally induced ischemic renal failure in dogs. Ten dogs were used in this study. All dogs underwent right nephrectomy and 60 minutes of left renal ischemia followed by reperfusion. The animals were divided into two equal groups. The control group received no treatment, and the O3 group received an ozone–oxygen mixture. All groups were evaluated clinically and biochemically until day 40 after surgery, after which they underwent histopathological assessment. Major ozonated autologous hemotherapy substantially reduced the degree of elevation in the treatment group’s serum blood urea nitrogen and creatinine levels relative to the control group. Medical ozone therapy considerably reduced the degree of glomerular filtration rate decline and notable alterations; it resulted in a morphological improvement in renal tubular structure compared with that in the control group, and a well-structured improvement in glomeruli, which were reasonably close to those in the healthy control group. According to the findings, ozone therapy may be an effective way to enhance renal function. However, our results indicate that the aforementioned treatment has potential as a straightforward therapeutic strategy for the management of renal failure that may be caused by ischemia or other factors.