PRODUCTION OF DOCOSAHEXAENOIC ACID (DHA) FROM Thraustochytrium sp. ATCC 26185 USING DIFFERENTS NITROGEN CONCENTRATIONS

Abstract

In this work it was studied the polyunsaturated fatty acids (PUFAs) production, especially DHA, from Thraustochytrium sp. ATCC 26185, under different total nitrogen (TN) availability. Three different TN conditions were evaluated: two with initial concentrations of
2.4 g/L and 0.8 g/L, and the third in a fed-batch process with a rate of 0.009 g/L.h. For each experiment the biomass, glucose, TN and PUFAs were determined. The major composition of the PUFAs in Thraustochytrium sp. ATCC 26185 cell biomass were DPA ω6
(21-25 %) and DHA (69-73 %), regardless of the type and time of culture. The maximum cell concentration (30.2 g/L) was obtained using 2.4 g/L TN in 168 h of culture. With this same concentration of TN it was possible to produce the highest concentration of DHA (1.16 g/L) in 120 h of culture, demonstrating that the growth of Thraustochytrium sp. ATCC 26185 and yield of PUFAs are dependent on the source concentration of TN available for consumption of this oleaginous microorganism, as well as culture time.