EVALUATING THE EFFECTS OF DIFFERENT CONCENTRATIONS OF CHLORINE ON THE ARTEMIA LARVAE HATCHING AND SHEDING (Artemia sp.)

Abstract

This paper was prepared in the Aquaculture Laboratory of the Federal University of the State of Parana, Brazil, with the purpose of determining the best sodium hypochloride (NaClO with 6% active ingredient) dosage to be employed in the artemia oocysts hatching process.The artemia oocysts were hydrated for 1 hour, filtered and submitted to a 10-minute chlorine treatment. The chlorine dosages tested were the following (NaClO - 6% sodium hypochloride): 0.38g, 0.75g, 1.50g, 3.00g and 6.00g of active ingredient per liter of water. Soon after chlorine application the oocysts were rinsed to eliminate the chlorine and immediately the oocysts were put into transparent plastic vials with water in the constant aeration and luminosity, where they remained for 53 hours. For the nauplii count one mililiter of the vial was put into a test tube with an interconnected aeration. Three counts were made for all repetitions and the threshold analysed was the number of hatched larvae per mililiter of hatching solution. The results were submitted to a normality test and it was determined that the hatching results did not show a normal pattern, therefore, it was not possible to use those averages as a comparison basis between treatments. Subsequently non-parametric tests were performed using the overall average as a comparison basis between treatments.Techically one can see the superiority of treatment with 1.50g of active chlorine, which resulted in the highest hatching-out counts, with 50% of the observed data presented 140 larvae hatched per milimiter.