IDENTIFICATION OF HOLSTEIN COWS CARRIERS OF COMPLEX VERTEBRAL MALFORMATION BY HIGH RESOLUTION MELTING CURVES (HRM)

Authors

  • Andrea Branda Sica Instituto Nacional de Investigación Agropecuaria (INIA), INIA Las Brujas, Unidad de Biotecnología
  • Paula Nicolini Universidad de La República, Centro Universitario de Tacuarembó, Instituto Superior de la Carne, Área Biología Molecular.
  • María Teresa Federici Instituto Nacional de Investigación Agropecuaria (INIA), INIA Las Brujas, Unidad de Biotecnología
  • Silvia Llambí Universidad de La República. Facultad de Veterinaria, Sección Genética.

DOI:

https://doi.org/10.5380/avs.v24i4.65494

Keywords:

Holstein, CVM, high-resolution dissociation curve.

Abstract

The objective of this study was the optimization and implementation of a reliable and economical molecular screening method for the detection of the mutant allele of CVM (complex vertebral malformation, c.559G>T, SLC35A3) by HRM analysis, as well as analyzing its existence in a representative sample of Holstein cows from the Milk Genomic DNA Bank of Uruguay. The optimization of the HRM methodology in the RotorGene™ 6000 equipment (Corbett Life Science, Australia) by amplification of the 79 bp PCR products clearly differentiated the two genotypes: homozygous, wild type: GG; and heterozygous, carrier for the mutation CVM: GT (c.559G>T; SLC35A3). In the analyzed sample, the frequency of the mutant allele (T) for CVM was high (q = 0.032), with a prevalence of carrier cows of 6.45%. It is concluded that the PCR-HRM analysis is a fast, easily interpretable, low cost, and highly accurate technique for the detection of this mutation in Holstein cattle, which may be implemented in genetic selection programs.

Author Biography

Andrea Branda Sica, Instituto Nacional de Investigación Agropecuaria (INIA), INIA Las Brujas, Unidad de Biotecnología

Unidad de Biotecnología

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Published

2019-12-31

How to Cite

Branda Sica, A., Nicolini, P., Federici, M. T., & Llambí, S. (2019). IDENTIFICATION OF HOLSTEIN COWS CARRIERS OF COMPLEX VERTEBRAL MALFORMATION BY HIGH RESOLUTION MELTING CURVES (HRM). Archives of Veterinary Science, 24(4). https://doi.org/10.5380/avs.v24i4.65494

Issue

Vol. 24 No. 4 (2019)

Section

Animal Reproduction

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