LABORATORY DIAGNOSIS AND CLINICAL SIGNS OF CANINE VISCERAL LEISHMANIASIS IN DOGS EXAMINED AT THE CENTER FOR ZOONOSIS CONTROL IN CAMPO GRANDE – MS, BRAZIL

Authors

  • Rodrigo Casquero Cunha Embrapa Beef Cattle
  • Renato Andreotti Embrapa Beef Cattle
  • Elaine Silva Center for Zoonosis Control in Campo Grande
  • Elisângela Pereira University of South Matogrosso
  • Tayra Sato Embrapa Beef Cattle
  • Vanete Thomaz-Soccol Positive University

DOI:

https://doi.org/10.5380/avs.v17i4.24951

Keywords:

ELISA, PCR, IFA, RV1/RV2 primers

Abstract

Visceral leishmaniasis is a type of zoonosis caused by several Leishmania species endemic to tropical, subtropical, and Mediterranean climate regions. Dogs are the primary source of infection in urban areas and can be symptomatic or asymptomatic. This study focused on the observation of clinical signs of leishmaniasis in dogs in Campo Grande, Mato Grosso do Sul, Brazil. Samples from affected animals were analyzed using indirect fluorescent antibody (IFA) tests, an enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays to determine the optimal diagnostic tool for use on animals that present clinical symptoms. A predominance of clinical symptoms affecting the integumentary system was observed, and splenomegaly and hepatomegaly were the most important pathological signs. Among the diagnostic tests, the greatest agreement was seen between ELISA and IFA, followed by ELISA and PCR, and finally IFA and PCR. PCR diagnostic results showed the greatest extent of correlation with clinical signs, followed by ELISA and then IFA. When choosing a diagnostic method, veterinarians should consider the clinical signs and health status of the patient.

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Published

2012-09-04

How to Cite

Cunha, R. C., Andreotti, R., Silva, E., Pereira, E., Sato, T., & Thomaz-Soccol, V. (2012). LABORATORY DIAGNOSIS AND CLINICAL SIGNS OF CANINE VISCERAL LEISHMANIASIS IN DOGS EXAMINED AT THE CENTER FOR ZOONOSIS CONTROL IN CAMPO GRANDE – MS, BRAZIL. Archives of Veterinary Science, 17(4). https://doi.org/10.5380/avs.v17i4.24951

Issue

Vol. 17 No. 4 (2012)

Section

Preventive Veterinary Medicine

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