BACTERIA AND INFLAMMATORY RESPONSE IN THE UTERUS OF BITCHES AFTER ARTIFICIAL INSEMINATION

Autores

  • Ana Paula Coelho Ribeiro Universidade Federal do Tocantins
  • Wilter Ricardo Russiano Vicente Universidade Estadual Paulista - Câmpus de Jaboticabal
  • Ivo Walter dos Santos Universidade Federal do Paraná - Câmpus de Palotina
  • Maricy Ferreira Apparício Universidade Estadual Paulista - Câmpus de Jaboticabal
  • Roberta Carareto Universidade Federal do Tocantins - Câmpus de Araguaína
  • Caroline Peters Pigatto Universidade Federal do Tocantins - Câmpus de Araguaína

DOI:

https://doi.org/10.5380/avs.v15i2.13263

Palavras-chave:

bitch, inflammation, reproduction, semen, cadela, inflamação, reprodução, sêmen

Resumo

The present study aimed to obtain information about the uterine inflammatory response (number of polymorphonuclear neutrophilic granulocytes - PMNs) in bitches after artificial insemination (AI) and identify the uterine microflora present after the following treatments: insemination using semen with extender (n=6), insemination with fresh semen (n=6) and no inseminated (n=6). The percentage of PMNs on the endometrial surface and within histological sections was evaluated together with the presence of aerobic bacteria in the uterine lumen. For endometrial cytology, there was no significative difference on the number of inflammatory cells between bitches not inseminated (3.05 ± 1.74 PMNs) and those inseminated with fresh semen (3.55 ± 1.51 PMNs); There was a significative difference in both groups compared to the inseminated with semen plus extender (7.80 ± 1.67 PMNs) (p<0.05). Histology showed that there was no significative difference on the number of inflammatory cells between bitches not inseminated (87.72 ± 35.2 PMNs) and those inseminated with fresh semen (122.97 ± 43.31 PMNs); however, it was observed  differences in both groups compared to those inseminated with semen plus extender (171.94 ± 42.74 PMNs) (p<0.05). Eight animals, randomly distributed in the groups, showed the presence of  Staphylococcus sp and Proteus sp., in the microbiological exam. The extender for semen, with Tris, is a potent inducer of uterine inflammation, and positive uterine cultures may be obtained during estrus without inflammation or uterine infection.

Biografia do Autor

Ana Paula Coelho Ribeiro, Universidade Federal do Tocantins

Professor Adjunto da disciplinas : Obstetricia Veterinária e Fisiologia da Reprodução e Insemnação Artificial . àrea de concentração: Reprodução aplicada à Obstetricia

Wilter Ricardo Russiano Vicente, Universidade Estadual Paulista - Câmpus de Jaboticabal

Professor Titular da Disciplina Obstetricia Veterinária - àrea: Obstetrícia

Ivo Walter dos Santos, Universidade Federal do Paraná - Câmpus de Palotina

Departamento de Reprodução Animal

Maricy Ferreira Apparício, Universidade Estadual Paulista - Câmpus de Jaboticabal

Doutorando em Reprodução Animal

Roberta Carareto, Universidade Federal do Tocantins - Câmpus de Araguaína

Professor Adjunto da Disciplina Anestesiologia Veterinária

Caroline Peters Pigatto, Universidade Federal do Tocantins - Câmpus de Araguaína

Professor Adjunto das Disciplinas Microbiologia Geral e Específica

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Publicado

2010-11-04

Como Citar

Ribeiro, A. P. C., Vicente, W. R. R., dos Santos, I. W., Apparício, M. F., Carareto, R., & Pigatto, C. P. (2010). BACTERIA AND INFLAMMATORY RESPONSE IN THE UTERUS OF BITCHES AFTER ARTIFICIAL INSEMINATION. Archives of Veterinary Science, 15(2). https://doi.org/10.5380/avs.v15i2.13263

Edição

Seção

Medicina Veterinária Preventiva