INFLUENCE OF THE CULTURE MEDIUM AND AGAR CONCENTRATION IN THE GROWTH AND DEVELOPMENT OF PLANTLETS COFFEE FROM DIRECT SOMATIC EMBRYOGENESIS
DOI:
https://doi.org/10.5380/rsa.v9i1.9900Keywords:
Coffea arábica, cultura de tecidos, melhoramento genéticoAbstract
An important method of in vitro propagation of Coffea arabica plants is somatic embryogenesis. This work aimed to achieve the most adequate culture medium formulation and agar concentration for the plantlets growth of Coffea. arabica L. cv. Rubi from direct somatic embryogenesis. The experiment was carried out at the Tissue Culture Laboratory of Federal University of Lavras, in 2005, in randomized block, with four replications, and factorial 4 x 5. It was evaluated the influence of different types of culture media (MS; Knudson; WPM; White) and different concentration agar (0; 2; 4; 6 and 8 g L-1). The experiment was maintained for 60 days in a growth room under light intensity of 32 µMol m-2 s-1, 25 ± 1 oC photoperiods of 16 hours. The characteristics evaluated were: number of leaves, length of the aerial part and fresh mass weight of the plantlets. The results indicated the WPM culture medium, presented larger efficiency for development plantlets of Coffea arabica. The concentration of 1,5 g L-1 of agar provided the best fresh mass weight as well as the biggest length of the aerial part. The largest number of leaves was obtained in the absence of agar.
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