CULTURE MEDIUM AND GROWTH REGULATORS IN VITRO BLACKBERRY MICROPROPAGATION

Abstract

The micropropagation of blackberry can generate virus-plants free and in a short space of time. Aiming to improve in vitro propagation techniques of blackberry, were tested different culture media and 6-belzilaminopurin (BAP) concentrations and indolbutiric acid (IBA). The first experiment consisted cv. ‘Brazos' inoculated in 3 different culture medium, combined with five BAP concentrations. As the second experiment consisted cv. Tupy inoculated in 4 culture medium, combined with five IBA concentrations. The pH of culture medium was adjusted for 5.8 before the addition of 6 g cm^-3 agar and the sterilization  of    121 ºC and 1 atm for 20 min. After the inoculation, the explants had been kept per 70 days, in a growth room with 25 ± 1 ºC, irradiance of 35 mmol m^-2 s^-1 and photoperiod of 16 h. The experiments entirely casualizated, using three explants per repetition and 12 buds per treatment. There were verified bigger number of ‘Brazos’ buds in culture medium MS. Length and number of roots of cv. Brazos had been stimulated in culture medium Roubelakis and MS added 0,5 mg dm^-3 of BAP. The formation of radicular system of this cv. was observed of buds in all culture medium studied, however better resulted of cv Tupy, was observed on the absence of IBA for the culture medium Knudson, NN and MS.