INFLUENCE OF CULTURE MEDIUM COMPOSITION AND EXPLANT TYPE ON MICROPROPAGATION OF THE ROOTSTOCK Prunus sp. ‘GxN-9’

Authors

  • Elizete Beatriz RADMANN Universidade Federal de Pelotas, Faculdade de Agronomia Eliseu Maciel, Pelotas (RS)
  • Valmor João BIANCHI Universidade Federal de Pelotas, Pelotas (RS)
  • Tatiane Medeiros SOUZA Embrapa Clima Temperado, Pelotas (RS)
  • José Carlos FACHINELLO Universidade Federal de Pelotas, Faculdade de Agronomia Eliseu Maciel, Pelotas (RS)
  • Roberto Pedroso de OLIVEIRA Embrapa Clima Temperado, Pelotas (RS)

DOI:

https://doi.org/10.5380/rsa.v10i2.13573

Keywords:

Produção de mudas, pessegueiro, in vitro, citocinina, vitrificação, nursering, peach, citokynin, vitrification

Abstract

The objective of the present work was to study the composition of different culture medium, BAP concentration and explants types on in vitro multiplication of the rootstock ‘GxN-9’, like the influence of this factors in the vitrification. Basal explants cultivated in QL and WPM medium had the better results regarding shooting percentage (80%). The shoot number per explants was also higher with basal explants when cultivating in QL medium (3 shoot a explant). The medium QL and WPM allowed better growth performance of formed shoots to both explants types. Nevertheless, basal explants cultivated in QL medium had 55% of vitrification, whereas explants from WPM medium did not show this abnormality. The lower level of salt concentration with 0.5 mg dm-3 BAP reduced vitrification, promoting an increase in shoot growth without affecting formation rates.

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Published

2009-03-09

How to Cite

RADMANN, E. B., BIANCHI, V. J., SOUZA, T. M., FACHINELLO, J. C., & de OLIVEIRA, R. P. (2009). INFLUENCE OF CULTURE MEDIUM COMPOSITION AND EXPLANT TYPE ON MICROPROPAGATION OF THE ROOTSTOCK Prunus sp. ‘GxN-9’. Scientia Agraria, 10(2), 095–101. https://doi.org/10.5380/rsa.v10i2.13573

Issue

Vol. 10 No. 2 (2009)

Section

Crop Science

License

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