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MICROSCOPIC EXAMINATION OF PIG MASSETER MUSCLE BY POOLED SAMPLE DIGESTION METHOD TO IDENTIFY THE PRESENCE OF TRICHINELLA LARVAE

Andrijanto Hauferson Angi, Fadjar - Satrija, Denny Widaya Lukman, Mirnawati - Sudarwanto, Etih Sudarnika

Abstract

Abstract: Infection with the nematode worm Trichinella spp causes the disease Trichinellosis which is zoonotic and occurs worldwide. Trichinellosis cases in Indonesia are rarely reported. Pigs are susceptible to Trichinella spp. infection. Examination of the presence of Trichinella sp. worms in pigs is not sufficient by simply looking at the clinical symptoms that appear, or through examination of feces, or blood. A definitive diagnosis for morphological and anatomical identification of Trichinella worms or their larvae must be made through microscopic examination. This study aimed to determine the presence of Trichinella worms in the Masseter muscle organs of pigs through microscopic examination. A cross-sectional study was conducted on 330 muscle (meat) samples of pigs in slaughterhouses in Kupang City. Muscle samples were laboratory examined for the presence of Trichinella larvae by pooled sample digestion (33 pooled from 330 muscle samples) where positive results were examined individually by compression method. The results showed that 5 pooled muscle samples were positive for Trichinella. Positive results from 5 muscle pooled samples were followed by microscopic examination through the compression method. The results of the examination using the compression method found that 3 samples out of 330 muscle samples (0.9%) contained Trichinella spiralis larvae. These results indicate the presence of Trichinella spiralis larvae in pigs slaughtered in Kupang City and could pose a threat to people who consume pork.
Keywords: Trichinella spp., cross-sectional study, pooled sample digestion, Compression Method

Keywords

Animal Diseases ; Zoonosis Diseases ; Veterinary Public Health

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DOI: http://dx.doi.org/10.5380/avs.v28i4.91862