Ovarian morphometry applied to the use of chemosterilant in bitches and queens

Resumo

Chemical sterilization as an alternative method to surgical castration, have been studied. However, considering females, it is necessary to know adequate volumes of chemosterilizer to be injected into the ovary and biological factors that influence ovarian volumetry. This study aimed to determine the volume of 20% calcium chloride solution diluted in 95% ethanol to inject into the ovary of bitches and queens. Furthermore, correlations between ovarian measures (length [L], width [W], height [H], and volume) and body mass, age, and the volume of injected chemosterilizer were analyzed. For this, 64 ovaries, collected after elective ovariohysterectomy, received an injection of the solution. Ovarian measures were performed pre-injection and post-injection, and volume was calculated using Lambert's empirical formula, the formula for an ellipsoid, and the formula for spherical prolate. The mean ± standard error of the chemosterilant volume injected in the ovary was 0.09 ± 0.02 mL for bitches and 0.10 ± 0.02 mL for queens; ovaries’ width obtained a strong correlation with the injected volume; age showed a correlation to chemosterilant volume for both species and ovarian volume of queens; and body mass showed a correlation to ovarian measurements, ovarian volume e injected volume for bitches. Lambert's empirical formula and ellipsoid formula were more reliable for this assessment and respectively demonstrate an increase of 33% and 24% in ovarian volume in bitches post-injection; and 27% and 22% in queens post-injection. The use of chemosterilant beyond the possible limits of the organ may leakage in the abdominal cavity of females.