In vitro maturation of domestic cat (Felis catus) oocytes
DOI:
https://doi.org/10.5380/avs.v11i1.5623Keywords:
maturação in vitro, ovócitos, felinos, in vitro maturation, oocytes, felineAbstract
The establishment of genome resource banks and the application of assisted reproduction has been proposed as auxiliary tools for preserving endangered species. In this study domestic cat oocytes were matured in vitro (IVM) as a model for defining efficient protocols for wild felid species. A total of 808 oocytes (390 grade I and 418 grade II) were collected from 43 domestic cat ovaries, with a mean number of 9 oocytes, per category, per animal. To assess the meiotic cycle phase, 156 oocytes were stained with DNA specific marker. Overall, 70% of the oocytes were considered mature (Metáfase II). The mean maturation rate for oocytes grade I (74±3%) were higher (p<0.05) than that for oocytes grade II (67±3). In conclusion, the described protocols were effective and domestic cat IVM can be used in our laboratory as a control for studies in wild felids.
Downloads
How to Cite
Issue
Section
License
Authors that wish to publish in AVS agree with the following conditions:
- To keep copyright of the article and allow the AVS to publish the first time. The article will be licensed by Creative Commons - Atribuição 4.0 Internacional allowing the sharing of their work.
- Authors may distribute their work by other channel of distribution (ex.: local or public repository).
- Authors have the permission to publish their work online, using different channels (similar to above), even before the final editorial process.
