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Total number of spermatozooa from stud-horses collected by means of artificial vagina and through feedback flow of the epididymis tail

L.C. GRANEMANN, R.R. WEISS, L.E. KOZICKI, P.R. MURADAS, T.E. TREML

Abstract


The aim of the present research work,
carried out with 7 stud-horses, was to compare the total
number of spermatozzoa recovered from the artificial
vagina. In a preliminary phase, evaluation of the semen
harvested with the artificial vagina was carried out. After 7
days of this procedure, the stallions underwent bilateral
orchiectomy for recovery and evaluation of the epididymal
tail spermatozooa. For the latter procedure, the spermatic
cells had been harvested through the segmentation in 3
parts of the epididymal tail and its washing through a
feedback flow with proper diluents. The counting of the
total number of spermatozoa was carried out immediately
after the procedures of their harvesting. It was concluded
that there was a statistical difference (p<0,05) between
the two used techniques.

Keywords


espermatozóides; garanhão; cauda do epidídimo; spermatozoids; stallion; tail of the epididymis



DOI: http://dx.doi.org/10.5380/avs.v11i1.5607