Association between the serologic analysis and the polymerase chain reaction (PCR) in the diagnostic of the Mycoplasma hyopneumoniae infection in pigs during quarantine procedure

Abstract

This study investigated through serologic analysis and polymerase chain reaction (PCR) the presence of Mycoplasma hyopneumoniae in reproductive gilts during quarantine proceeding. This study was realized in 300 Landrace x Large White gilts with 150 days of age in a quarantine unity in Parana in 2007.  These animals came from a single farm, considered free from Mycoplasma hyopneumoniae. The exams were made at Microvet laboratory located in Viçosa-MG. Clinical signs that suggest of respiratory problems were not seen, nor seroconversion was detected in 130 serologic analysis, performed in serum samples collected on days 0, 55 and 76 after housing, however, the Mycoplasma hyopneumoniae was detected through PCR (649 bp fragment of 16S gene) in tracheobronchiolar wash and pulmonary tissue from a gilt without conclusive result in serologic analysis in a sample collected 55 days after housing. Hence, association between serologic analysis and PCR was important to diagnose Mycoplasma hyopneumoniae during quarantine proceedings.