Open Journal Systems

Hepatoprotective treatment improves leukocyte responses of Wistar rats intoxicated by CCl4

Marco Antonio de Andrade Belo, Luciano Melo de Souza, Vando Edésio Soares, Márcia Ferreira da Rosa Sobreira, Daniela Myasaka Silveira Cassol, Sandra Barioni Toma


The aims of this investigation was to evaluate the effect of hepatoprotective treatments with a compound

prepared by the association of N-Acetyl DL-Methionine (5%) + Choline chloride (2%) + Caffeine (1%) +

Thiamine hydrochloride (1%) + Nicotinamida (0,5%)+ Pyridoxine hydrochloride (0.04%), administered

through intramuscular (IM) route, at doses of 0.2, 0.6 and 1.0 mL/kg of BW, through the study of leukocytes

responses in rats submitted to acute intoxication with CCl4. 147 females were randomized into 21 groups,

performing five different treatments, which were evaluated seven animals in four periods: two, four, six and

eight days after CCl4-induced intoxication. In this study, it was observed absolute eosinophilia and

monocytosis in animals untreated and treated with the lowest dose of 0.2 mL. These responses were

significantly better in animals treated with 0.6 and 1.0 mL/ kg BW. The untreated animals showed

thrombocytopenia, when compared to treated animals. Absolute neutropenia and lymphocytosis was

observed in all rats intoxicated with CCl4, there is no difference among treatments. The analysis of white

blood cells demonstrated that the hepatoprotective treatments favored the leukocyte response, by act

beneficially on the population of these cells, supporting the hypothesis that these events may reduce the

deleterious effects in liver tissue after intoxication by CCl4.


hepatoproteção; leucócitos; ratos; tetracloreto de carbono