Comparative study between two environments for cultivation of Corymbia citriodora in vitro

Abstract

This research aimed to compare growth and development of C. citriodora shoots in JADS and MS environments measuring the total dry weight, total proteins, and total soluble sugars. The experimental design employed was randomized blocks, arranged in bifactorial model, with timing split plots with three replications per treatment. Treatments: T1 (control, 40 ml of medium per bottle, without addition during the assessment), T2 (40 ml of medium per bottle adding 10 ml just on third day of assessment), T3 (40 ml of medium per bottle adding 10 ml on third and fourth days of assessment) as subplots, seven assessment periods (0, 3, 6, 9, 12, 15, 18). As result, for the medium JADS it is recommended two additions of culture medium during the explants development. For MS it is not indicated add cultivating environment during C. citriodora development.