Morphology of Eucalyptus urophylla callus, cultivated in vitro under boron and calcium concentrations

Abstract

Boron and calcium are essential nutrients in plant development, but there are few registers of the common action of these nutrients in vegetable. In order to identify and quantify the possible implications of these micro and macronutrients in the morphogenetic development of Eucalyptus urophylla, callus were cultivated in vitro with different concentration of boron (0, 25, 50, 100 e 200 µM H₃BO₃) and calcium (0, 3.75, 7.50, 11.25 e 15.00 mM CaCl₂). The experiment was completely randomized design in factorial arrangement (5 x 5) with 5 replications. It was evaluated after 21^th and 31^th days of culture the relation of external and internal morphology. It could be verified that in two evaluations periods the combined of boron and calcium had intervened in aspects of the internal e external morphology that had caused direct answers in visual aspects of the calluses. The combinations of 25 µM B with 0, 3.75, 7.50, 11.25 e 15.00 mM Ca in 21^th day, were less harmful of the roots favoring appropriately organ development. The production of dry matter weight was directly influenced by boron concentration and this effect was constant for all calcium concentration and for both evaluated days.