IN VITRO ESTABLISHMENT OF BLUEBERRY TREES STARTING FROM NODAL SEGMENTS

Authors

  • Alan Cristiano ERIG
  • Márcia Wulff SCHUCH

DOI:

https://doi.org/10.5380/rsa.v6i1.4603

Keywords:

cultura de tecidos, meio de cultura, 2iP, pequenas frutas, tissue culture, WPM, culture medium, small fruits

Abstract

With the objective of defining the culture medium constitution, the explant length and the physical state of culture medium to favor the in vitro establishment of blueberry, cvs. Flórida and Delite, were accomplished two experiments. In the experiment I, where nodal segments of the cv. Flórida were used as explants, it was studied four different constitutions of the WPM culture medium (T1: WPM - testimony; T2: WPM + 24.6 µM 2iP; T3: WPM + 24.6 µM 2iP + 2.68 µM NAA; and, T4: WPM + 24.6 µM 2iP + 2.68 µM NAA + 1.44 µM GA3). The culture mediuns with Gamborg vitamins, were added of myo-inositol (100 mg.L-1), sucrose (30 g.L-1) and agar (6 g.L- 1). In the experiment II, with vegetable material of the cv. Delite, were studied two physical states of the culture medium (semi-solid and liquid) and three explants lengths - nodal segments (0.5; 1 and 1.5 cm). In this experiment, the WPM culture medium with Gamborg vitamins was added of 2iP (24.6 µM), myo-inositol (100 mg.L-1) and sucrose (30 g.L-1). To the semi-solid medium agar was added (6 g.L-1), and in the liquid medium, cotton wads sustained the explants. In the experiment I, the WPM culture medium + 24.6 µM 2iP propitiated the higher establishment percentage (70.74%). In the experiment II, the length of the explant did not have effect on establishment. In relation to the physical state of the culture medium, the establishment percentage was higher being used the semi-solid medium (79.23%), compared to the liquid medium (0.74%).

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How to Cite

ERIG, A. C., & SCHUCH, M. W. (2005). IN VITRO ESTABLISHMENT OF BLUEBERRY TREES STARTING FROM NODAL SEGMENTS. Scientia Agraria, 6(1), 91–96. https://doi.org/10.5380/rsa.v6i1.4603

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Section

Scientific Articles