NITROGEN SOURCES IN COFFEE MICROPROPAGATION

Authors

  • Lilian de Sousa RIBEIRO
  • Moacir PASQUAL
  • Anna Lygia de Rezende MACIEL
  • Evaldo de Sousa ARANTES
  • Edvan Alves CHAGAS

DOI:

https://doi.org/10.5380/rsa.v3i1.1039

Keywords:

cafeeiro, segmento nodal, cultura de tecidos, meio MS, Coffee plant, nodal segment, tissue culture, MS medium.

Abstract

Considering that the MS medium is very rich in mineral salts ally to the high cost and the difficulty in the acquisition of nitrate and ammonium it was aimed at to reduce the concentration of two sources of nitrogen (NH4NO3 and KNO3) used in the culture medium of nodal segments of Coffea arabica L. cv. Rubi. Explants (2 cm) preestablished in vitro were inoculated in MS medium with NH4NO3 and KNO3 (0, 25, 50, 75 and 100 %) and maintained at growth room with 27±1 ºC temperature and 16 hours/day under 32 mM.m-2.s-1 light intensity. After 60 days were evaluated the total number of sprouts, the aerial part fresh matter weight and the total dry matter weight. Its possible to reduce the NH4NO3 concentration to 75% and KNO3 to 50%.

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How to Cite

RIBEIRO, L. de S., PASQUAL, M., MACIEL, A. L. de R., ARANTES, E. de S., & CHAGAS, E. A. (2002). NITROGEN SOURCES IN COFFEE MICROPROPAGATION. Scientia Agraria, 3(1), 107–112. https://doi.org/10.5380/rsa.v3i1.1039

Issue

Vol. 3 No. 1 (2002)

Section

Scientific Articles

License

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