Aspectos anatômicos da cultura in vitro da Araucaria angustifolia. II. 0 enraizarnento dos brotos axilares
DOI:
https://doi.org/10.5380/abpr.v22i0.717Palavras-chave:
axillary-shoots, micropiopagation, rooting, Araucaria, angustifolia, micropropagação, enraizamento, botanyResumo
Foram estudados os aspéctos anatômicos da estrutura do calo do processo do enraizamento dos brotos axilares da Araucaria angustifolia. Estes brotos, em meio básico de Murashigue & Skoog, com a concentração dos nutrientes minerais reduzida à metado 1-2 mg de ácido indol-3-butírico. formam calo rizogênico somente nas regiões muito próximas da base. 0 excedente é do tipo friável, provavelmente dificultando a iniciação radicial. Entretanto. as raízes formadas são morfo e fisiologicamente funcionais.
Abstract
Anatomical aspects of the callus and the in vitro rooting process on axiliary shoots of Araucaria angustifoia were performed. These shoots cultivated in Murashigue & Skoogs basal medium at the half strength of mineral nutrients and 1-2 mg of indol-3-butyric acid, showed firmly stablished and rhizogenic callus only at the proximal sites of its bases. The excedent were friable callus, probably affecting the root primordia initiation. However, developed roots are phisiologicalty and morphologically functional.
Abstract
Les aspects anatomiques de la structure du cal et de la rhizogénese in vitro de pousses axillaires dAraucaria angustifolia ont été étudiés. Cés pousses cultivées sur le milieu de Murashigue & Skoog dans une concentration des minéraux réduit a 1/2, plus 1-2 mg de Lacid 3- indolybutirique, forment des cals compacts rhizogénes seulement a la proximité de leurs bases. Lexcedant étaient du tipe friable, possiblement impechent linitiation des racines. Les racines formées sur les cals compacts, sont morphologique et physiologiquement functionelles.
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