ISOLATION AND CHARACTERIZATION OF CARBENDAZIM DEGRADING Trichoderma harzianum RIFAI MUTANTS

Autores

  • ITAMAR SOARES DE MELO Empresa Brasileira de Pesquisa Agropecuária - SP
  • CÉLIA MARIA MAGANHOTTO DE S. SILVA Empresa Brasileira de Pesquisa Agropecuária - SP
  • JANE L. FAULL London University - UK

DOI:

https://doi.org/10.5380/pes.v20i1.20452

Resumo

Mutants of Trichoderma harzianum Rifai, obtained after ultraviolet (UV) light exposure, showed high resistant to the fungicide benomyl. A mutant (2B6) was capable of degrading carbendazim, other fungicide of the benzimidazole fungicide. This mutant degraded 41.5% of the molecule within five days. This and others mutants (2B1 and 2B2) presented variation in size and frequency of uni-nucleated and/or bi-nucleated spores compared to the wild type. Four primers generated RAPDs patterns that allowed the mutant to be differentiated from the wild-type. It is concluded that using UV mutagenization, it is feasible to obtain strains of T. harzianum with improved pesticide degradation ability.

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DE MELO, I. S., DE S. SILVA, C. M. M., & FAULL, J. L. (2010). ISOLATION AND CHARACTERIZATION OF CARBENDAZIM DEGRADING Trichoderma harzianum RIFAI MUTANTS. Pesticidas: Revista De Ecotoxicologia E Meio Ambiente, 20. https://doi.org/10.5380/pes.v20i1.20452

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